Figure 7. BMSC soluble factor(s) inhibits mENT1 activity and protect APL cells from AraC-induced apoptosis.

(A) Measurement of mENT1 activity. APL cells were cultured alone or with M2-BM SN for 24 hours. Cells were harvested and mENT1 activity was measured by the incorporation of radioactive ³H-adenosine (B) RNA extraction from APL cells cultured alone or in presence of M2-BM SN for a time-course of 0, 3, 6, 9, 15, and 24 hours was used for RT-PCR to detect expression of mENT1. (C) Western blot analysis for mENT1 using APL cells with or without M2-BM SN for 24 hours. Each bar represents the mean ± SD of 3 independent experiments. ***p<0.001 (APL versus APL SN).