Skip to main content
. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Anal Chem. 2011 Oct 27;83(22):8484–8491. doi: 10.1021/ac2017037

Table 1.

Protease digestion preparation conditions for human apo-transferrin.

Reaction
Conditions		 Denaturing
Agent		 Reducing
Agent		 Alkylating
Agent		 Quenching
Agent
#1a 0.1% RapiGest™ 5 mM DTT 15 mM IAA
#2a 6 M Urea 5 mM DTT 15 mM IAA
#3a 0.1% RapiGest™ 10 mM DTT 15 mM IAA 20 mM DTT
#4a 0.1% RapiGest™ 5 mM TCEP 10 mM IAA
#5a 6 M Urea 10 mM DTT 15 mM IAA 20 mM DTT
#6a 6 M Urea 5 mM TCEP 10 mM IAA
#7b 6 M Urea 5 mM TCEP 10 mM IAA 10 mM DTT
a

Initial reaction conditions tested.

b

Reaction condition predicted as optimal after analysis of conditions 1 through 6.