. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: Anal Chem. 2011 Oct 27;83(22):8484–8491. doi: 10.1021/ac2017037

Table 2.

Transferrin peptides detected for seven different protein digestion reaction conditions.

Reaction Conditions	^a#1	^a#2	^a#3	^a#4	^a#5	^a#6	^b#7

Protein Coverage	51.1 %	62.0 %	69.8 %	63.8 %	74.6 %	70.8 %	75.9 %
Cys Containing Peptides Detected	47.4 %	63.2 %	66.7 %	69.2 %	76.3 %	68.4 %	76.3 %
Under Alkylation Detected	0.0 %	0.0 %	13.8 %	0.0 %	0.0 %	6.5 %	0.0 %
Over Alkylation Detected	34.8 %	38.5 %	3.4 %	33.3 %	0.0 %	9.7 %	0.0 %
Type of Glycans Detected at N⁴³²	^cBi	Bi	Bi	Bi	Bi	Bi	Bi
Type of Glycans Detected at N⁶³⁰	Bi	Bi	Bi	Bi	Bi	Bi	Bi/^dTri

Initial reaction conditions tested.

Reaction condition predicted as optimal after analysis of conditions 1 through 6.

Bi = Biantennary N-linked Glycans.

Tri = Triantennary N-linked Glycans.