Skip to main content
. 2012 May 2;32(18):6061–6071. doi: 10.1523/JNEUROSCI.0221-12.2012

Figure 1.

Figure 1.

A, B, Ensembles of complex output LCNs resolved by anti-GFP labeling of the GAL4 lines NP5092 (A) and NP3045 (B). A, Left, Hemisection through the brain labeled with anti-α-tubulin and anti-GFP, showing the ensemble of type Col A LCN neurons in the lobula with converging axons to its corresponding Col A glomerulus. This lies ventral and medial to a glomerulus receiving terminals of neurons with dendrites in both the lobula plate and the lobula (LPL neurons), belonging to the morphological type LPL2CN neurons resolved by anti-GFP labeling of the GAL4 line NP5092. An individual recorded and dye-filled neuron of this ensemble is shown in Figure 5A. Right, Reconstruction of 14 of the 24 glomeruli, most of which are in the inferior lateral protocerebrum (bracketed), each supplied by an ensemble of columnar output neurons from the lobula complex (lobula plate and lobula). The depth shown here, from the level of the FB, is ∼40 μm. Glomeruli are color coded according to depth and are identified from serial vertical sections of brains labeled by “tricolor” labeling (elav-GAL4 > UAS-DsRed, UAS-n-syb::GFP, UAS-Rdl-HA) to resolve presynaptic and postsynaptic densities, glia, and other aspects of neuropils and their connections (see http://flybrain.iam.u-tokyo.ac.jp/flydb091226/php/flydb/index.php). Two anterior glomeruli, receiving inputs from the Col A and LPL2CN neurons, lie in front of a more posterior glomerulus supplied by L1CN neurons (see B). FB, Fan-shaped body of the central complex; LH, lateral horn; LO, lobula; LOP, lobula plate; ME, medulla; PED, pedunculus of the mushroom body; SP superior protocerebrum. Abbreviations and terms follow standard nomenclature for the Drosophila brain. B, Palisade ensemble of the lobula columnar output neuron L1CN, resolved by anti-GFP labeling of the GAL4 line NP3045. Somata (bracketed), from which patch-clamp recordings were obtained, reside in a uniquely identifiable location above and medial to the lobula upper margin. Left, Columnar neurons after section-by-section image deletion of other profiles expressing GFP. Right, All profiles resolved at this level before removal. Scale bars, 50 μm.