Figure 3. KSR1 is required for immune synapse localization of phosphorylated stathmin.
CD4+ T cells from KSR1 wild-type or knockout AND TCR mice were stimulated with CH57 Bcells loaded with 1μg MCC peptide for 5 days until rested. T cells were purified and conjugated with CH27 cells loaded with peptide. Conjugates were stained with phospho-S24 stathminantibody and analyzed by confocal microscopy. (B) Graph represents 3 experiments of >20 conjugates each. Scale bar = 10 μm. P-value <0.001 (Two-tailed unpaired t-test).