Figure 6. DCs from Abcg1^−/− mice selectively induce the differentiation of Th2 cells.

(A) Whole cell lysates prepared from sorted naïve splenic DCs were subjected to SDS-PAGE and immunoblotted for ABCG1 and β–Actin. (B) CD103⁺ and CD11b⁺ DCs sorted from the lungs of naïve Abcg1^+/+ or Abcg1^−/− mice were pulsed ex vivo with OVA in the presence of LPS, and then co-cultured with naïve CD4⁺ T cells isolated from spleens of OT-II mice for 5 days. IL-4, IFN-γ, and IL-17A were quantified in culture supernatant by ELISA. Experiments were performed in triplicate wells and data is presented as mean +/− SEM. Representative of 3 independent experiments. *, p<0.01; **, p<0.001.