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Dimerization of single-cysteine RNase by QSOX. 1C65-RNase (200 μM) was incubated in phosphate buffer at 25 °C with (triangles) or without (circles) 500 nM HsQSOX. Samples were withdrawn for measurement of thiol titer and for analysis by non-reducing SDS-PAGE after quenching residual free thiols using NEM (see Methods and inset to the main figure).
