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Interaction between Dansyl-1C65-RNase and PDI or TbQSOX. Dansyl-1C65-RNase (0.5 μM in phosphate buffer) was titrated with the concentrations of oxidized PDI shown (open circles; see the Text) and the increase in dansyl fluorescence was fit to a Kd value of 3.9 ± 0.4 μM (see Methods). A comparable titration with 1 μM Dansyl-RNase was conducted with TbQSOX (closed diamonds) and shows a negligible change of fluorescence.
