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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 23;188(11):5604–5611. doi: 10.4049/jimmunol.1200039

Figure 4. TLR2−/− deficient mast cells exhibit increased F. tularensis replication and cellular destruction.

Figure 4

(A) WT and TLR2−/− mast cell analysis by confocal microscopy. Lucifer yellow labeled LVS (green); c-Kit (purple) surfaced labeled mast cells with intracellular MHCII (light blue), LAMP (orange), and acidification probe (red). Representative images shown at 30 min; 1000X magnification. (B) LVS (green) infected WT and TLR2−/− mast cell analysis by confocal microscopy at 3 h; c-Kit (purple), LAMP (orange), MHCII (light blue). (C) Bacterial enumeration (F. tularensis SCHU S4) of cellular lysates and active caspase-3 analysis by flow cytometry at 3 (white) and 24 h (solid bar). Unpaired t test *p < 0.05. Error bars represent the mean ±SD.