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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 30;188(11):5528–5537. doi: 10.4049/jimmunol.1102629

Figure 2.

Figure 2

The immune response in mice with altered DNA polymerase ζ function. (A–B) Rev3 knock-out B cells experienced a decrease in the frequency of mutations at Ig V regions during the immune response to NP-CGG compared to WT mice and CD19 transgenics. Close to 40% of the clones in the Rev3 knock-out had 0–1 mutations, compared to less than 10% in the controls. Spleens from Rev3 knock-out mice displayed normal GC with 46% of the clones achieving the affinity-enhancing W to L mutation at position 33 of Vh186.2. (C) Rev3 knock-in mice (Rev3L2610F) had a similar overall distribution of mutational classes as controls but had a higher percentage of clones with more than 9 mutations. These mice displayed normal GC, but also displayed an increase in the fraction of Vh186.2 clones with the affinity enhancing W to L change (pie chart). The following mice were used for these experiments: C57BL/6 and CD19 promoter-driven cre recombinase transgenics in the C57BL/6 background, n = 6 mice and 75 unique clones; Rev3 knock-out, n = 6 mice and 59 unique clones; Rev3L2610Fclones, n = 4 mice and 50 unique clones. Unique clones were determined by the CDR3 amino acid sequence.