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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 20;188(11):5257–5266. doi: 10.4049/jimmunol.1101934

Figure 3. Humoral responses to TI-2 antigens are decreased during chronic T3-SCI.

Figure 3

(A) Experimental design for NP-Ficoll immunization: 28 days post-SCI or post-laminectomy, mice were immunized with NP-Ficoll and the splenic tissue was analyzed at day 4.5. (B) Representative FACS gating strategy to identify NP-specific B cells. Dot plots show the frequency of Aqua−CD4−CD8−F4/80−Gr1−Igκ−Igλ+NP+ responding B cells among live singlets. (C) Total number of NP-specific B cells. (D) Total number of NP-specific IgM ASCs. For all data sets, n=5 for un-injured, n=7 for T9-Lam, n=6 for T9-SCI, n=7 for T3-Lam, and n=8 for T3-SCI. ** denotes significance at p<0.01.