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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 20;188(11):5257–5266. doi: 10.4049/jimmunol.1101934

Figure 5. Germinal center B cells and high-affinity ASCs are reduced in T3-injured mice after TD immunization.

Figure 5

(A) Representative FACS plots for identification of NP-specific GC B cells in un-injured mice (CD19+CD4−CD8−F4/80−Gr1−IgD−PNA+NP+Fas+). Numbers shown in each dot plot are percentages of each population within the parent gate. (B) The absolute number of GC B cells in the spleen (n=4–5 mice per group). (C) Total number of high-affinity NP-specific IgG1 ASCs in BM and spleen. (D) Serum concentrations of NP-specific high-affinity IgG1 antibody above non-immunized controls. (E) Affinity maturation within the spleen as determined by the ratio of high-affinity to total IgG1 ASCs (Figure 4D); For C–E, n=5 for uninjured, n=5 for T9-SCI, and n=9 for T3-SCI. Data are representative of two experiments. ** denotes significance at p<0.01 and * denotes significance at 0.01≤p<0.05.