A. Q-PCR quantitation of two epithelial markers, E-cadherin and CLDN3. Addition of T3 receptor antagonist TRA reduces the activation of E-cadherin and CLDN3 by T3 treatment. 293 cells were used as the positive control.
B–C. Down-regulation of Snail1 and Snail2, two mediators of the epithelial-to-mesenchymal transition.
D. Morphological changes of the reprogrammed cells in the presence or absence of T3.
E. Immunostaining of E-cadherin expression in reprogrammed cells in the presence or absence of T3. Note that the T3 receptor antagonist TRA abolishes the T3-mediated activation of E-cadherin.