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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Mol Immunol. 2012 May 1;51(3-4):347–355. doi: 10.1016/j.molimm.2012.04.001

Fig. 5.

Fig. 5

Phosphatidylcholine levels and ER abundance in LPS-stimulated wild-type and ATF6α-deficient B cells. Splenic B cells were isolated from Atf6a+/+ and Atf6a−/− mice and cultured in the presence of LPS for 3 days. (A) Lipids were extracted from equivalent numbers of cells and the amount of phosphatidylcholine (PtdCho) was determined. The experiment was performed in duplicate using B cells from two mice of each genotype; data are plotted as mean ± S.D. of triplicate determinations. (B) Cells were left unstained (hashed lines) or stained with ER-Tracker™ (solid lines) and analyzed by flow cytometry for ER abundance. Data are from a representative experiment out of two repeats.