Figure 6.
Suppressive capability of Tregs from PTPN22 deficient mice. CD4+ CD25+ Tregs were isolated from splenocytes from WT, Het and KO mice using magnetic separation. 3 × 104 CFSE labeled Teff (CD4+ CD25−) T cells were cultured in the presence of 1 × 105 irradiated splenocytes and soluble anti-CD3 antibody (1μg/ml). Tregs were titrated into the cultures at the ratios indicated. The cells were cultured for 72 hours and then proliferation was assessed by flow cytometry. This experiment is representative of 3 separate experiments. The 96 hour timepoint shows similar results from 2 separate experiments (data not shown).