Table I.
MHC binding capacity of mouse peptides recognized by islet-infiltrating HLA-restricted T cells in HLA-transgenic NOD mice and their human equivalents
| A: HLA-A*1101-transgenic NOD mice | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Binding to A3 supertype members (IC50, nM) | |||||||||
| Organism | Position | Sequence | A*1101 | A*0301 | A*3001 | A*3101 | A*3301 | A*6601 | A*6801 |
| Mouse | IGRP 272–280 | AINSEMFLRa | 209 | 428 | 49752 | 67 | 1081 | 4547 | 109 |
| Human | IGRP 272–280 | AINSEMFLL | 2824 | 1258 | 6776 | 1071 | 12784 | 36672 | 14186 |
| Mouse | Ins1 C21-30 | TLALEVARQK | 3327 | 605 | 23280 | 13026 | 25455 | – b | 13432 |
| Mouse | Ins2 C23-32 | TLALEVAQQK | 833 | 856 | – | – | – | – | 7494 |
| Human | Ins C23-32 | PLALEGSLQKc | 37072 | 20873 | – | 39403 | – | 48509 | 41456 |
| Mouse | Ins1 C22-31 | LALEVARQKR | – | 43592 | 42979 | 10808 | 9145 | 12966 | 12582 |
| Human | Ins C24-33 | LALEGSLQKR | 34461 | 17521 | – | 38387 | 3271 | 515 | 66 |
| B: HLA-B*0702-transgenic NOD mice | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Binding to B7 supertype members (IC50, nM) | |||||||||
| Organism | Position | Sequence | B*0702 | B*3501 | B*3503 | B*4201 | B*5101 | B*5301 | B*5401 |
| Mouse | Ins1 C15-24 | SPGDLQTLAL | 4.4 | – | 16115 | 35 | – | 10987 | – |
| Human | Ins C15-26 | GPGAGSLQPLAL | 462 | – | – | 6066 | – | 15456 | – |
a
bold and underlined letters indicate amino acid identity between murine and human peptides
b
dash indicates IC50 > 50000 nM
c
recently reported as an HLA-A3-restricted epitope in humans (26)