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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 20;188(11):5682–5693. doi: 10.4049/jimmunol.1103760

Figure 5. Co-incubation with C1q tails or inhibition of type I IFNs reduces the induction of IL-27 by C1q in LPS-stimulated HMDMs.

Figure 5

HMDMs were incubated with EAL, C1q-EAL, LAL or C1q-LAL at a 5:1 ratio for 1 h (A–B) in presence of C1q tails and then stimulated with 10 ng/ml LPS for 3 h or (C–E) stimulated with LPS for 18 h in presence of 1 µg/ml control mouse IgG1 or neutralizing antibodies against IFNα and/or IFNβ (C–D) or goat IgG or neutralizing antibodies against IL-27 (E). mRNA levels were determined by qRT-PCR and proteins levels by ELISAs. Results represent means ± s.d. (n = 2–3 different donors, performed in duplicates), two-way ANOVA, * p < 0.05, ** p < 0.01 and ***, p < 0.001.