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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Dev Biol. 2012 Apr 20;366(2):195–203. doi: 10.1016/j.ydbio.2012.03.020

Figure 2.

Figure 2

Morpholino-mediated gene knockdown of sema3d and its putative receptors. In all graphs, MO represents the particular gene-targeting morpholino; MM represents the particular 5 mis-match/control morpholino; UN represents uninjected/untreated fins. (A) Total regenerate length was measured. sema3d-knockdown and plxna3-knockdown cause reduced fin length (*); nrp2a-knockdown causes increased fin length (*). (B) The total number of H3P positive cells were counted. sema3d-knockdown causes reduced levels of cell proliferation (*); nrp2a-knockdown causes increased levels of cell proliferation (*). (C) Segment length was measured in treated wild-type fins. sema3d-knockdown and plxna3-knockdown cause reduced segment length (*). (D) Segment length was measured in treated alf dty86 fins. sema3d-knockdown and plxna3-knockdown cause reduced segment length and rescue joint formation in alf dty86 (*). Statistically different data sets (*) were determined by the student’s t-test where p < 0.05. By the student’s t-test, there is no statistical difference between MM and UN for any mismatch morpholino. Error bars represent the standard deviation.