Representative Western blots and quantitative analysis of SphK1 (A), SphK2 (B), phosphorylated Akt (Ser473) (p-Akt; C), and cleaved caspase-3 (D) in the left cerebral hemisphere at 24 hours after SAH (study 1). The p-Akt band density values are calculated as a ratio of that of total Akt, and the other proteins band density values are calculated as a ratio of that of β-actin. Values are mean±SD; *P<0.05, ANOVA.