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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 25;188(11):5785–5791. doi: 10.4049/jimmunol.1200109

Fig. 2. Phenotypic change of T cells in PC61-injected mice.

Fig. 2

B6 mice were randomly separated into two groups (n=8) and immunized with IRBP1–20/CFA with or without a prior treatment with PC61. After 13 days, splenic T cells were enriched and stimulated with the immunizing peptide. The phenotype of the T cells was analyzed either before (A–C) or after (D–F) in vitro stimulation.

A–C) Effect of PC61 injection on CD25+CD3+ cells (A), Foxp3+ cells (B), and γδ T cells (E).

D–F) After in vitro stimulation with the immunizing antigen and expansion under Th17 polarized conditions, the activated T cells were stained with the indicated antibodies and analyzed by flow cytometer. The experiments were repeated more than 5 times and the results of one representative experiment are shown.