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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 25;188(11):5785–5791. doi: 10.4049/jimmunol.1200109

Fig. 5. Restoration of the Th17 response by addition of activated γδ T cells to functionally defective T cells.

Fig. 5

A–C). In vivo primed T cells from IRBP1–20/CFA-immunized mice with (B&C) or without (A) prior treatment with PC61 were stimulated for 5 days with immunizing peptide under Th1 or Th17 polarized conditions (A and B) or after addition of 2% of activated γδ T cells (2 × 104/well), then activated T cells were intracellularly stained for IFN-γ and IL-17 and analyzed by FACS for numbers of IL-17+or IF-Nγ+T cells.

D) IFN-γ and IL-17 levels in the culture supernatants at 48 h were assessed by ELISA.