Skip to main content
. 2012 Jun;18(6):1210–1221. doi: 10.1261/rna.031997.111

FIGURE 2.

FIGURE 2.

The L123I mutation from the Euplotes and Blepharisma YCF motifs reduces eRF1 recognition of the UGA codon. (A,B) Fold change in readthrough by viable (A) and inviable (B) strains expressing the indicated mutant eRF1 proteins compared with wild-type S. cerevisiae eRF1. The data are expressed as mean values (relative to wild-type eRF1) ± standard deviation. The horizontal dashed lines represent wild-type S. cerevisiae eRF1 levels. (C,D) Western blots showing steady-state eRF1 proteins levels from strains assayed in A and B, respectively.