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Introduction of the Loxodes TASNIKS and/or YCF motifs reduce UAA/UAG recognition by S. cerevisiae eRF1. (A,B) Fold change in readthrough by strains expressing the indicated mutant eRF1 proteins compared with wild-type S. cerevisiae eRF1. The data are expressed as mean values (relative to wild-type eRF1) ± standard deviation. The horizontal dashed lines represent wild-type S. cerevisiae eRF1 levels. (C,D) Western blot showing steady-state eRF1 proteins levels from strains in A,B.
