FIGURE 3.
VapC proteins Rv0065, Rv0617, PAE0151, PAE2754 display sequence-specific, Mg2+-dependent ribonuclease activity. VapCPAE0151 and VapCPAE2754 appear to target the same sequence as seen by the same RNA fragments on the gels; this is also seen for VapCRv0065 and VapCRv0617, which also appear to target the same sequence. Negative controls (RNA only at 0- and 60-min time points) show no ribonuclease contamination; 12 mM EDTA (E) inhibits VapC activity, and VapC when in complex with VapB (C) displays no ribonuclease activity. This lane is missing from the VapCPAE2754 panel as VapBC cannot be produced in this case due to the insolubility of VapB. Ladder indicates sizes of RNA.