Figure 5.

Dependency of alignment accuracy on read coverage. Alignment accuracy with respect to read coverage is shown for the mouse data set with 1% error rate: (A) complete reads and (B) junction reads. For this purpose, genes were binned into four approximately equal-sized groups based on the average read count per base and accuracy was calculated selectively for each group. For complete reads, no dependency was observed, whereas for junction reads, accuracy is strongly dependent on the read coverage.