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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunother. 2012 Jun;35(5):430–439. doi: 10.1097/CJI.0b013e31825898e8

Figure 2. IL-12 secreting cells monitored by ELISPOT assay.

Figure 2

Human PBLs were stimulated by OKT3 (50ng/ml) and sequentially transduced with a TCR recognized MAGE-A3 and three NFAT.hIL12 γ-retroviral vectors separately. The transduced cells were with PMA (10 ng/ml)/Ionomycin (2.2 μM) and after 16 hours, the IL-12 specific spots were imaged and counted by immunoSpot Micro analyzer (left). The numbers of spots were determined (right) from three wells and values presented represented the mean + SD. M, MAGE-A3 TCR.