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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunother. 2012 Jun;35(5):430–439. doi: 10.1097/CJI.0b013e31825898e8

Figure 5. Young TIL transduced with NFAT.IL12 vector clone D3 produce IL-12 production upon antigen recognition.

Figure 5

The CD8 enriched TILs were subjected to REP (day 0) and transduced with clone D3 and GFP vector on day 4. Two days later, the transduced cells were co-cultured with antigen-positive and HLA-A2+ tumor lines: mel526, mel624, and HLA-A2-tumor lines: mel888 and mel938. The level of IL-12 (A) and IFN-γ B in the cultures was measured by ELISA (shown are the mean values of triplicate determinations, +/− standard deviation). (C) The fold expansion of transduced cells was calculated by counting viable cell number on day 14.