Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 3;67(6):477–487. doi: 10.1136/thoraxjnl-2011-200508

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.

PMC Copyright notice

Increased urokinase plasminogen activator receptor (uPAR) expression leads to attenuated wound repair. Cells transfected with pc-uPAR show elevated expression by (A) Western blotting with control (uPAR), (B) immunofluorescence and (C) quantitative PCR 24 h after transfection (n=5). Increased soluble uPAR (suPAR) (D), decreased urokinase plasminogen activator (uPA) (E) and no change in plasminogen activator inhibitor 1 (PAI-1) (F) levels were observed in supernatants. Changes in matrix metalloproteinase (MMP)-9 and MMP-2 levels/activity were detected using (G) zymography and (H) an ELISA-based assay. (I) Cells overexpressing uPAR showed attenuated wound repair. Control cells were treated with supernatants from cells containing pc-DNA3 or pc-uPAR at wounding (J) or 24 h prior to wounding (K) and repair was measured (n ≥4, two donors). *p<0.05, **p<0.01, ***p<0.001.