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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Anal Biochem. 2012 Mar 30;425(2):175–182. doi: 10.1016/j.ab.2012.03.018

Figure 2.

Figure 2

Verification of Western blot system linearity. Linearity of our system was examined by loading five different amounts of total protein (0.125, 0.25, 0.5, 1.0, and 2.0 μg) in triplicate from a MHC IIa sample, and evaluated for GAPDH content. The GAPDH signal (Integrated Density Value = IDV) was plotted against corresponding amounts of total protein.