Skip to main content
. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Anal Biochem. 2012 Mar 30;425(2):175–182. doi: 10.1016/j.ab.2012.03.018

Figure 3A & B.

Figure 3A & B

Western blot GAPDH load and transfer evaluation. (A) Single MHC I and MHC IIa samples were loaded (1 μg total protein) in quintuplicate (CV = 11% and 4%, respectively). MHC IIa fibers displayed 96% more GAPDH when compared to MHC I fibers. (B) Single MHC I and MHC IIa samples were loaded in triplicate at 1 μg and 2 μg of total protein and assessed for GAPDH content. GAPDH protein content doubled when 2 μg was compared to 1 μg. MHC IIa fibers again displayed 123% and 98% more GAPDH when compared to MHC I fibers at 1 and 2 μg, respectively. MHC = myosin heavy chain. CV = coefficient of variation.