CS content across the continuum of human skeletal muscle fiber types. Lanes were loaded with 3 μg of total protein as follows: MHC I/IIa, MHC IIa, MHC IIa/IIx, MHC IIx, and MHC I. MHC I, MHC I/IIa, MHC IIa, and MHC IIa/IIx displayed 528%, 472%, 242%, and 47% greater content of CS when compared to MHC IIx, respectively. Due to sample limitation, only one CS fiber type continuum experiment was performed and therefore SE values are not available. MHC = myosin heavy chain. CS = Citrate Synthase.