Figure 3.

Inhibition of attachment of MC3T3 cells to fibronectin-coated discs by the HFN7.1 antibody and the effect of pretreatment with heat (HEAT) and with radiofrequency plasma glow discharge (RFGD). Untreated and pretreated discs were coated overnight with 1 nM (0.44 μg/ml) fibronectin, incubated with cells for 2 h, and the number of (A) MC3T3 or (B) MG63 cells that attached to coated discs was measured in the presence of various dilutions of the HFN7.1 anti-fibronectin Ig. The number of cells that attached to these discs at each antibody dilution was measured, corrected for background cell attachment, and then expressed as a percentage of the attached cell number (corrected for background) measured for coated discs in the absence of antibody. The background cell attachment is the number of cells that attached in the absence of both the antibody and the fibronectin coating. In experiments using the HFN7.1 antibody, data for untreated discs were obtained from four to six independent cell cultures for each antibody dilution, data for HEAT-pretreated discs were obtained from six independent cell cultures, and data for RFGD-pretreated discs were obtained from three to four independent cell cultures. Data from three independent cultures of MC3T3 cells are also presented for the IST-4 anti-fibronectin Ig that was tested as a negative control for experiments using untreated discs only. AB, antibody. Data represent mean ± SE. * > untreated group at the corresponding antibody dilution (p < 0.05) based on ANOVA.