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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Eur J Oral Sci. 2012 May 8;120(3):185–194. doi: 10.1111/j.1600-0722.2012.954.x

Figure 5.

Figure 5

Effects of pretreatment with heat and with radiofrequency plasma glow discharge (RFGD) on the binding of anti-integrin Igs to integrin receptors that became attached to adsorbed fibronectin during incubation with MC3T3 cells. Untreated and pretreated discs were coated overnight with various concentrations of fibronectin and then incubated with MC3T3 cells for 2 h. The binding of (A) an anti-α5 integrin Ig, (B) an anti-β1 integrin Ig, or (C) an anti-α2 integrin Ig to cross-linked integrins was quantified as shown in Figure 4. The relative fluorescence intensity (RFI) measurements obtained at each indicated coating concentration of fibronectin (1 nM corresponds to 0.44 μg/ml of fibronectin) are presented. Data for untreated discs were obtained from three to six independent cell cultures for each antibody dilution and data for heat-pretreated and RFGD-pretreated discs were obtained from three independent cell cultures. Data represent mean ± SE. * > untreated group at the corresponding fibronectin-coating concentration (p < 0.05) based on ANOVA.