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. 2012 Apr 16;109(19):7257-7262. doi: 10.1073/pnas.1116971109

Fig. 2.

Fig. 2.

Amorfrutins selectively regulate gene expression in adipocytes. (A) Structure of the PPARγ:amorfrutin 1 complex. PPARγ binds to amorfrutin 1 between helix H3 (red) and the β-sheet (green). The C-backbone of amorfrutin 1 is drawn in yellow and oxygens in red. (B) Atomic details of ligand recognition. Hydrogen bonds stabilizing the complex are shown as dashed lines, experimental amorfrutin 1 electron density is shown in gray. (C) Gene distance matrix of gene expression profiles in human adipocytes treated with amorfrutin 1 or 2 (30 μM), rosiglitazone, pioglitazone, nTZDpa (10 μM each) or telmisartan (30 μM). Squares show the distance of two compounds in Euclidean space, ranging from exactly the same profile (black) to completely different (red). (D) Venn diagram of differentially expressed genes after treatment of human adipocytes with different compounds. Numbers in circles indicate up- and down-regulated genes, numbers in parentheses represent a total of regulated genes for that compound. (E) Enriched pathways after treatment of human primary adipocytes using GSEA. Ten most highly significant pathways for amorfrutin 1 and corresponding normalized enrichment scores (NES) are shown. #, x, $, P ≤ 0.05 for amorfrutin 1, amorfrutin 2 or rosiglitazone.