Fig. 5.

RNAi knockdown of peroxisome biogenesis genes decreases endogenous peroxide level and increases oxidative stress tolerance. (A) Reduced H₂O₂ levels in pex1 and pex13 mutant flies. **Significant (P < 0.01) changes compared with the corresponding WT flies (w¹¹¹⁸). (B and C) Decreased endogenous ROS levels (B) as measured by DCF-DA (Materials and Methods) and enhanced resistance to paraquat (C) in worms with RNAi knockdown of prx-5, prx-11, prx-13, or F18F11.1. Asterisks indicate significance of difference compared with the EV control worms (*P < 0.05, **P < 0.01).