Fig. 1.

Efficiency of Pt-GG bypass by hPol η. (A) Structures of a thymine dimer (CPD) and a Pt-GG lesion. (B) Base selection and TLS efficiency of hPol η in four consecutive steps of bypassing a Pt-GG. DNA oligos used in the assays are shown above each gel. The results with normal DNA of the same sequences are shown for comparison. Reactions were carried out with a single type or a mixture of all four dNTPs as indicated at a total concentration of 0.1 mM. (C) A plot of relative polymerase efficiency (k_cat/K_M) of single nucleotide incorporation in each step of Pt-GG bypass normalized to the average efficiency with normal DNA as summarized in SI Appendix, Table S2.