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Serum inhibits IAV entry into GnT1-deficient cell lines. (A–D) Entry of luciferase-expressing IAV [x-axis, virus stock (microliters); y-axis, relative light units] into the WT CHO cell lines k1 (A) and Pro5 (C) and their derivative GnT1-deficient cell lines 15B (B) and lec1 (D) was studied in the absence (black bars) or the presence (gray bars) of 10% FCS. (E and F) Entry into cells pretreated with NA (VCNA) demonstrates that entry in all cells was strictly dependent on sialic acid.
