Fig. 5.
Other examples of biologically induced ripening. (A) Volume evolution of a droplet containing E. coli in M9 medium supplemented with 0.1 mM CaCl2, 1 mM MgSO4, 1 g/L casamino acid, and 45 g/L of glucose (pH = 7.4). After a small increase in size, the droplet shrinks to half its initial volume within 30 h. (Insets) Transmission images of droplets at times indicated by red arrowheads. (Scale bars: 25 μm.) (B) Volume evolution of a droplet containing 0.2 mM of BSA, in Tris/HCl buffer (pH = 7.5) in the presence of 40 μg/mL of protease K. As the BSA is digested by the protease, the droplet inflates to reach twice the initial volume. (Insets) Transmission images of droplets at times indicated by red arrowheads. (Scale bars: 40 μm.)