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. Author manuscript; available in PMC: 2012 May 23.
Published in final edited form as: Cell Stem Cell. 2008 Jun 5;2(6):553–565. doi: 10.1016/j.stem.2008.03.020

Figure 2. Perinatal grafts of human glial progenitors yield widespread and dense host myelination.

Figure 2

A–B. Serial sagittal images of an engrafted shi/shi x rag2−/− brain, sacrificed at 1 year of age. Each image in a and b represents a montage of 50–100 images at 10×. Each series begins 750 μm lateral to the midline, and continues at 600 μm intervals. A. human donor cells, immunolabeled in 14 μm cryosections using an anti-human nuclear antibody (hN; red). B. Alexa 488-labeled myelin basic protein (MBP; green) in sections adjacent or nearly so to their matched sections in A. All major white matter tracts, including those of the corpus callosum, capsules, striatum, fimbria, cerebellum and brainstem heavily express MBP.

C–G. Black-and-white images of MBP-immunoreactive fibers in a number of sites reveal high efficiency axonal myelination; all images of transplanted shi/shi x rag2−/− mice at > 1 year post-transplant. C, The rostral striatum, corpus callosum, and neocortical layers 5 and 6 are shown in sagittal section. D. Higher magnification of c shows the MBP-defined myelination of individual fibers within the striatum, as well as the larger bundles of corticostriatal and striopallidal fibers. E. Donor-myelinated MBP+ fibers in a longitudinal section of the cervical spinal cord; dorsal column to the left, central gray to the right. F. Interwoven donor-myelinated fibers of the brainstem, in the pontine base. G. Donor-derived MBP in the conus medullaris; exiting myelinated roots of the cauda equina to the left.

Scale: A, B = 2.5 mm; C = 200 μm; D = 40 μm; E = 50 μm; F = 60 μm; G = 125 μm