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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Anal Biochem. 2012 Mar 23;425(2):128–134. doi: 10.1016/j.ab.2012.03.012

FIGURE 6.

FIGURE 6

MSP analysis of sFRP1 and TFPI2 genes in colon samples using the rapid and classic DNA extraction. Methylation pattern of (A) sFRP1 and (B) TFPI2 gene promoter region in 18 FFPE colon samples using rapid DNA extraction method. Colon FFPE tumor samples labeled 1 to 18 were extracted by a rapid method (R) and a classic phenol-chloroform method (C). For DNA samples extracted by classic method, we presented two representative samples (C10 and C11). Also, HCT 116 (colon cancer cell line) and DKO (DNMT1 and DNMT3b double knock out in HCT 116 cell) were extracted by classic phenol-chloroform method. PCR products recognizing unmethylated (U) and methylated (M) CpG sites were analyzed on 2% agarose gels visualized with GelStar nucleic acid gel stain (Cambrex Bio Science). IVD, in vitro methylated control; H2O, water control containing no DNA.