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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Bone Miner Res. 2011 Jul;26(7):1425–1436. doi: 10.1002/jbmr.345

Fig. 3.

Fig. 3

Effects on gene expression of acute exposure of mature NHBC cultures to exogenously added SCL. NHBCs were cultured under mineralizing conditions for 35 days. Cells then were cultured for a further 3 or 7 days in the absence or presence of rhSCL at 1, 10, or 50 ng/mL. The medium and supplements were replenished on day 3. Total RNA was prepared and real-time RT-PCR was performed to determine mRNA expression of (A) OCN, (B) COL1α1, (C) E11, (D) TNAP, (E) DMP1, (F) SOST, (G) LRP4, (H) PHEX, and (I) MEPE. Data shown are means of triplicate reactions ± SD normalized to expression of GAPDH mRNA. a, b, and c indicate significant differences (p <.05) from untreated controls for rhSCL at 1, 10, and 50 ng/mL, respectively. Near-identical results were obtained from three independent experiments using NHBCs from different donors.