Figure 1.

Blood collection from the jugular veins of ICR albino and C57BL pigmented mice. (A) By applying petroleum jelly to fur wetted with alcohol, the jugular vein was visible as a blue, pulsating area. By using a syringe, a 27-gauge needle was inserted into the jugular vein through the pectoral muscle. (B) Hair around the thoracic region was removed by shaving. Blood sampling was performed by using a syringe with a 27-gauge needle. (C) Cutting the syringe with a plastic tubing cutter. (D) The collected blood sample was transferred into a capillary tube and then centrifuged.