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Time course assay of halofuginone inhibition. Halofuginone (1 μM) was added to HepG2 cells at the indicated times after P. berghei sporozoite addition (−2, 2, 6 12, and 24 hours). Parasite load in HepG2 cells was assessed by the relative luminescence signal 45 hours post-infection. Luminescence values were normalized to the DMSO control and reported as the average percent parasite load ± the standard deviation.
