Figure 7. STHdh Q111/Q111 cells expressing expanded Htt form stress granules more robustly than normal Q7/Q7 cells. (A) STHdh Q7/Q7 (top) and Q111/Q111 (bottom) cells were treated with 10 μM thapsigargin for 50 min before fixing, to induce ER stress. Caprin-1 and G3BP1 were detected as described in the legend to Figure 7. (B) Graph shows percentage of Q7/Q7 and Q111/Q111 cells containing stress granules (Caprin-1 and G3BP1- positive) upon treatment with 10 μM thapsigargin for 50 min before fixing, as determined by the presence of yellow dots in the merged images as shown in A. Total 150 cells were counted for each cell line (*n = 3, p = 0.02).