Figure 2. E2F1 interacts with APC/C components Cdh1 and APC5 in vivo. HEK293 cells were transfected with plasmids expressing HA-E2F1 and APC5 (A) or GFP-Cdh1 (B), and then treated with MG132 as indicated. (A) HA-tagged E2F1 was immunoprecipitated from cell lysates with HA beads and the co-immunoprecipitated APC5 was detected by immunoblotting. (B) GFP-Cdh1 was immunoprecipitated from cell lysates using GFP antibody and the co-immunoprecipitated E2F1 was detected by immunoblotting.