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. Author manuscript; available in PMC: 2012 May 23.
Published in final edited form as: Nat Protoc. 2010 Jun;5(6):993–1004. doi: 10.1038/nprot.2010.48

Figure 7.

Figure 7

Total ion chromatograms of unsulfated saturated chondroitin oligosaccharides up to 16-mers in negative ion mode separated by means of RPIP-HPLC. The inset shows the ESI-MS/MS spectra of the chondroitin disaccharide. The nomenclature for disaccharide fragmentation proposed by Domon and Costello52 is also illustrated. Fragment ions that contain a nonreducing terminus are labeled with uppercase letters from the beginning of the alphabet (A,B,C), and those that contain the reducing end of the oligosaccharide are labeled with letters from the end of the alphabet (X,Y,Z). Subscripts indicate the cleaved ions. A and X ions are produced by cleavage across the glycosidic ring, and are labeled by assigning a number to each ring bond and counting clockwise. Ions produced from cleavage of successive residues are labeled Am, Bm and Cm, with m = 1 for the nonreducing end, and Xn, Yn and Zn, with n = 1 for the reducing-end residue. Modified from reference 42.