Figure 4.

Schematic representation of the expression cassettes in pELipA^a (a), pELipB1^a and pELipB3^a (b), pELipB1^k and pELipB3^k (c), pELipAB^a (d), pELipA-B1^a and pELipA-B3^a (a,b), pELipAB-B1^a and pELipAB-LipB3^a (d,b), pLipAB^a + pELipB1^k and pLipAB^a + pELipB1^k (d,c), derived from vector pET22b(+) and pET28a(+) for E. coli BL21. T7Pro, T7 promoter; PelB, PelB signal peptide; lipA, mature lipase gene; His, 6xHis tag; T7Ter, T7 terminator; ΔlipB, truncated lipB ORF encoding a LipB derivative lacking the first 56 or 26 amino acids, corresponding to LipB1 and LipB3; lipB, the complete chaperone gene.