Table 2.
Production of the active lipase LipA by the dual expression cassette plasmid systems BL21/pELipAB-LipB1a and BL21/pELipAB-LipB3a
| Expression system BL21/ | Culture supernatant | Whole cell lysate | Whole culture | Cell lysate's supernatant | Cell lysate's precipitate | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| U/g | % | U/g | % | U/g | % | U/g | % | U/g | % | |
| pELipABa | 99.9 ± 1.5 | 100 ± 1.5 | 127.5 ± 1.1 | 100 ± 0.8 | 227.4 ± 0.9 | 100 ± 0.4 | 32.4 ± 1.6 | 21.3 ± 1.1 | 119.7 ± 8.6 | 78.7 ± 5.6 |
| pELipAB-LipB1a | 5064.7 ± 11.2 | 5068.4 ± 11.2 | 1408.3 ± 6.9 | 1104.5 ± 5.4 | 6473.0 ± 18.0 | 2846.1 ± 7.9 | 267.9 ± 9.6 | 17.2 ± 0.6 | 1287.9 ± 49.4 | 82.8 ± 3.2 |
| pELipAB-LipB3a | 2930.8 ± 4.5 | 2932.9 ± 4.5 | 1352.1 ± 4.2 | 1060.4 ± 3.3 | 4282.9 ± 8.5 | 1883.1 ± 3.7 | 192.0 ± 7.0 | 11.8 ± 0.4 | 1437.9 ± 47.1 | 88.2 ± 2.9 |