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. 2012 Jan 31;13:21. doi: 10.1186/1471-2105-13-21

Figure 6.

Figure 6

Nuclear segmentation in IHC tissue image analysis. Extracted nuclei (middle) can be used for quantitative IHC analysis such as the percentage or intensity levels of positively stained nuclei (right), where gray areas are positively nuclei and blue areas are negatively stained nuclei.