Table 1.
Variations within genes related to central carbon, nitrogen and sulfur metabolism
| Gene locus in NRRL 2338 | Length (amino acids) | Gene/locus | Variationa | Protein function and notes |
|---|---|---|---|---|
| Central carbon and energy metabolism | ||||
| SACE_0618 | 286 | ccdA | Missense (G38S) | Cytochrome c biogenesis protein |
| SACE_0633 | 221 | citA | IPM | Citrate synthase (N-terminal) |
| SACE_1638 | 609 | sucB | Missense (V574A) | 2-oxoglutarate dehydrogenase complex E2 component (dihydrolipoamide succinyltransferase) |
| SACE_3073 | 375 | hypD | Nonsense (Y30*) | Hydrogenase expression/formation protein HypD |
| SACE_5291 | 335 | dhaK | Frameshift (-C 717) | Dihydroxyacetone kinase N-terminal containing protein (DhaK) |
| SACE_6118 | 1125 | pyc | Frameshift (-C 1222) | Pyruvate carboxylase |
| SACE_6385 | 1206 | kgd (sucA) | Missense (1773 S) | 2-oxoglutarate dehydrogenase complex El component |
| SACE_6668 | 288 | sucD | In frame insertion (-2081, -209V, -210M, -2111, -212G, -213E) | Succinyl-CoA synthetase, alpha subunit |
| Nitrogen and amino acid metabolism | ||||
| SACE_0635 | 122 | ureB | Frameshift (-C 352) | Urease beta subunit |
| SACE_3800 | 689 | Missense (P601L) | Assimilatory nitrate reductase catalytic subunit (selenocysteine-containing) | |
| SACE_4319 | 426 | aspB | Missense (Y185C) | Aspartate aminotransferase |
| SACE_5299 | 433 | Missense (G103R) | D-amino acid deaminase | |
| SACE_5427 | 417 | Frameshift | D-amino acid dehydrogenase, small | |
| SACE_5741 | 500 | gltD | Frameshift (-G 1301) | Glutamate synthase NADH/NADPH, small subunit |
| SACE_6330 | 421 | Frameshift (-G 324) | Fusion protein (ligase/carboxylase and argininosuccinate lyase) | |
| SACE_6764 | 378 | Missense (T47A) Frameshift (-C 436) | Alanine racemase | |
| Sulfur and amino acid metabolism | ||||
| SACE_3346 | 517 | Missense (C286R) | Sulfite oxidase/oxidoreductase, molybdopterin binding | |
| SACE_4434 | 269 | tauC | Missense (L221F) | Taurine transport system permease protein TauC |
| SACE_4651 | 279 | tauD | Missense (G177S) | Taurine dioxygenase, 2-oxoglutarate-dependent |
| SACE_6133 | 158 | cdo2 | Missense (P151S) | Cysteine dioxygenase |
| Sugar metabolism | ||||
| SACE_0652 | 383 | iroB | Missense (A325V) | Glycosyl transferase, related to UDP-glucuronosy transferase |
| SACE_1940 | 788 | lacZ2 | Missense (A769V) | Beta-galactosidase/beta-glucuronidase |
| SACE_3069 | 207 | gmhA | Frameshift (-G 429) | Phosphoheptose isomerase |
| SACE_3071 | 223 | gmhA | Frameshift (-C 75,-C 90, -C91, -T 95, -G 98, -G 99, -C 100, -G 126) | Phosphoheptose isomerase |
| SACE_3737 | 1001 | ama2 | Missenses (R121A,N122Q, T123A, F124L, I125H, V127R) | Alpha-mannosidase |
| SACE_4596 | 814 | Missense (L810Q) | Beta-galactosidase/beta-glucuronidase | |
| SACE_5147 | 1099 | embC | Nonsense (S399*) | Arabinosyl transferase |
| SACE_5208 | 1098 | embC | Missense (S799F) | Arabinosyl transferase |
| SACE_5734 | 657 | Frameshift (+A 1027/1028) | Galactofuranosyl transferase | |
| SACE_6416 | 201 | rmlC | Missense (A173T) | dTDP-4-dehydrorhamnose 3,5-epimerase |
| SACE_6564 | 328 | deoC | Missense (G16S) | Deoxyribose-phosphate aldolase |
| SACE_6765 | 501 | Missense (R207G, P208R) | Ribokinase | |
| SACE_6971 | 433 | Missense (S23F) | Glycosyl transferase | |
| Fatty acid metabolism | ||||
| SACE_0351 | 281 | estC | Missense (A211T) | Esterase |
| SACE_3132 | 275 | abmC | Missense (V246G) Frameshift (-C 742) | Enoyl-CoA hydratase/isomerase (rev hydr) Putative: 2-ketocyclohexanecarboxyl- CoA hydrolase (amino benzoate degradation) |
| SACE_3216 | 526 | Frameshift (-G 1366) | Long-chain acyl-CoA synthetase | |
| SACE_3361 | 329 | Missense (T312I) | Glycerophosphoryl diester phosphodiesterase | |
| SACE_3450 | 304 | IPM | Enoyl-CoA hydratase/isomerase | |
| SACE_3478 | 568 | IPM | Long-chain acyl-CoA synthetase | |
| SACE_3745 | 255 | Missense (L187R) | Enoyl-CoA hydratase/isomerase | |
| SACE_3936 | 246 | Missense (L48H) | 3-oxoacyl-[acyl-carrier protein] reductase | |
| SACE_4589 | 388 | Frameshift (+CACC 10/11) | Acyl-CoA dehydrogenase | |
| Nucleotide metabolism | ||||
| SACE_1282 | 360 | nrdB | Missense (S2N) | Ribonucleotide-diphosphate reductase subunit β |
| SACE_2080 | 432 | pyrC | Missense (A90V) | Dihydroorotase |
| SACE_6664 | 521 | purH | Missense (P54S) | Bifunctional phosphoribosylaminoimidazolecarboxa mide formyltransferase/IMP cyclohydrolase No paralogue. |
| SACE_7125 | 538 | purF | Missense (P497S) | Glutamine phosphoribosylpyrophosphate amidotransferase No paralogue |
| SACE_2398 | 165 | prsA | Missense (T123P) | Phosphoribosylpyrophosphate synthetase |
| SACE_5196 | 506 | codA | Missense (Y322S) | Cytosine deaminase |
| Vitamin and cofactor metabolism | ||||
| SACE_0506 | 376 | thiO | Missense (A44V) | Amino acid oxidase flavoprotein ThiO, putativeGlycine/D-amino acid oxidases |
| SACE_0511 | 547 | thiC | Missense (T30I) | Thiamine biosynthesis protein ThiC |
| SACE_5955 | 1201 | cobN | Missense (A1091T) | Cobaltochelatase subunit CobN |
| Monooxygenases/oxidoreductases/methyltransferases/hydrolases of unknown function | ||||
| SACE_0651 | 422 | cypA | Frameshift (+C 16/17; +C 22/23) | Cytochrome P450 |
| SACE_0781 | 191 | Missense (P80S) | NADPH-dependent FMN reductase | |
| SACE_4325 | 341 | Frameshift (-C 890) | Radical SAM family protein Fe-S oxidoreductase | |
| SACE_4560 | 326 | Frameshift (+A 58/59) | SAM-dependent methyltransferase | |
| SACE_4563 | 515 | Missense (S9F) | Coproporphyrinogen III oxidase | |
| SACE_4854 | 366 | Frameshift (+G 477/478) | Amine oxidase, flavin-containing protein | |
| SACE_5012 | 454 | Missense (G114E) | Haem peroxidase | |
| SACE_5030 | 372 | Frameshift (-G 553) | Oye family NADH-dependent flavin oxidoreductase | |
| SACE_5053 | 229 | Missense (L204F) | Amidohydrolase 2 | |
| SACE_6588 | 491 | Frameshift (-C 633) | Monooxygenase, FAD-binding | |
| SACE_7243 | 462 | Nonsense (W188*) | FAD-dependent oxygenase/FAD/FMN-containing dehydrogenase | |
aIPM, imperfect match